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No additional information concerning myocyte renewal in these samples was obtained to support this approach.If the descending limb of the Relation between atmospheric 14C and time.The age of cardiomyocytes in these cases can be derived from both the ascending and the descending limb of the 14C curve (red ovals).This is the consequence of the degenerate function shown in Figure 1B.However, in retrospective C is used as the independent variable and time as the dependent variable (Figure 1B).This transformation produces a degenerate function, that is, one independent variable yields more than one dependent variables.By measuring only the percentage of troponin I–positive myocyte nuclei without considering the increase in fibroblasts, their data are consistent with the notion that the percentage of troponin I–positive myocyte nuclei increases with age.By necessity, the intensity of labeling of nuclei varied across the thickness of the section, becoming progressively weaker with the depth of the section.

These limitations apply also to labeling of the pericentriolar material-1 protein used to identify myocyte nuclei.

B, The birth date of noncardiomyocytes was not directly measured by C in cardiomyocytes and in the left ventricular myocardium.

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